Fig. 1: Fluorescence micrographs of staining patterns produced by DAPI (a, c, e and g) and the prototype human anti-CENP-F serum (b, d, f and h). CENP-F staining of interphase cells (a) is characterized by intense reactivity in a subpopulation of cells (b). Cells with maximal signal are in G2 phase of the cell cycle, since mixing of CENP-F and sera with anticentrosome antibodies illustrates centrosome duplication (arrows). In mitotic cells (e and f) there is punctate staining in the region of the metaphase chromatin. In telophase (g and h) there is intense staining in the midbody region of the segregating cells. Original magnification × 2500.
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