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Fig. 1: The oxyhemoglobin dissociation curve. The solid line represents O2 binding affinity to the hemoglobin molecule at standard temperature (37°C) and pH (7.4). The dashed lines represent hypothetical shifts in the curve: to the right with increased 2,3-diphosphoglycerate (2,3-DPG) levels or decreased temperature or pH; to the left with decreased 2,3-DPG or increased temperature or pH.