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Vol.
6 No. 1
Ribosome formation from subunits
studied by stopped-flow and Rayleigh light scattering
Ayman Antoun, Michael Y. Pavlov, Tanel Tenson and Måns
Ehrenberg
[Abstract] [Full Text] [PDF]
Negative Staining and Image
Classification – Powerful Tools in Modern Electron Microscopy
Melanie Ohi, Ying Li, Yifan Cheng and Thomas Walz
[Abstract] [Full Text] [PDF]
Phosphopeptide mapping of proteins
ectopically expressed in tissue culture cell lines
Beth A. Firulli, David M. Virshup and Anthony B. Firulli
[Abstract] [Full Text] [PDF]
Experimental modulation of
capsule size in Cryptococcus neoformans
Oscar Zaragoza and Arturo Casadevall
[Abstract] [Full Text] [PDF]
Force and Compliance Measurements
on Living Cells Using Atomic Force Microscopy (AFM)
Ewa P. Wojcikiewicz, Xiaohui Zhang and Vincent T. Moy
[Abstract] [Full Text] [PDF]
Vol.
5 No. 1
A Method to Identify p62’s
UBA Domain Interacting Proteins
Julia W. Pridgeon, Thangiah Geetha and Marie W. Wooten
[Abstract] [Full Text] [PDF]
Tumor cell-collagen interactions:
Identification and semi-quantitative evaluation of selectively-expressed
genes by combination of differential display- and multiplex-PCR
Rosalia Sirchia, Valentina Ciacciofera and Claudio Luparello
[Abstract] [Full Text] [PDF]
T Cell Integrin Overexpression
as a Model of Murine Autoimmunity
Raymond L. Yung, Donna Ray, Ru Ran Mo and Jun Chen
[Abstract] [Full Text] [PDF]
A screen to identify drug resistant
variants to target-directed anti-cancer agents Mohammad
Azam, Tal Raz, Valentina Nardi, Sarah L. Opitz and George Q.
Daley
[Abstract] [Full Text] [PDF]
Development of a new protocol
for 2-day generation of mature dendritic cells from human monocytes
Bianca Obermaier, Marc Dauer, Jan Herten, Katharina Schad, Stefan
Endres and Andreas Eigler
[Abstract] [Full Text] [PDF]
Swarming populations of Salmonella
represent a unique physiological state coupled to multiple mechanisms
of antibiotic resistance Wook Kim and Michael G. Surette
[Abstract] [Full Text] [PDF]
Effector cell mediated cytotoxicity
measured by intracellular Granzyme B release in HIV infected
subjects Supriya D. Mahajan, Ravikumar Aalinkeel, Stanley
A. Schwartz, Ram P. Chawda and Madhavan P.N. Nair
[Abstract] [Full Text] [PDF]
Use of surface plasmon resonance
for the measurement of low affinity binding interactions between
HSP72 and measles virus nucleocapsid protein Xinsheng
Zhang and Michael Oglesbee
[Abstract] [Full Text] [PDF]
Methods designed for the identification
and characterization of in vitro and in vivo
chromatin assembly mutants in Saccharomyces cerevisiae
Troy A. A. Harkness, Terra G. Arnason, Charmaine Legrand
and Ashley Lone
[Abstract] [Full Text] [PDF]
Dissecting mitosis by RNAi
in Drosophila tissue culture cells Helder Maiato, Claudio
E. Sunkel and William C. Earnshaw
[Abstract] [Full Text] [PDF]
Two-dimensional DNA displays
for comparisons of bacterial genomes Chad Malloff, Edie
Dullaghan, Alice Li, Richard Stokes, Rachel Fernandez and Wan
Lam
[Abstract] [Full Text] [PDF]
Application
of 4,5-diaminofluorescein to reliably measure nitric oxide released
from endothelial cells in vitro Thomas R. Räthel,
Jürgen F. Leikert, Angelika M. Vollmar and Verena M. Dirsch
[Abstract] [Full Text] [PDF]
Shotgun
Phage Display - Selection for Bacterial Receptins or other Exported
Proteins Karin Jacobsson, Anna Rosander, Joakim Bjerketorp
and Lars Frykberg
[Abstract] [Full Text] [PDF]
An
Application of Molecular Genotyping in Mice Lara A. Underkoffler,
Joelle N. Collins, Jonathan D. Choi and Rebecca J. Oakey
[Abstract] [Full Text] [PDF]
Measuring
Solution Viscosity and its Effect on Enzyme Activity Salvador
Uribe and José G. Sampedro
[Abstract] [Full Text] [PDF]
Methodology
for Detecting Trace Amounts of Microchimeric DNA from Peripheral
Murine White Blood Cells by Real-Time PCR Carol M. Artlett,
C. Gennaro Dito and Paul J. Christner
[Abstract] [Full Text] [PDF]
Immunological
assays for chemokine detection in In - vitro culture of CNS cells
Supriya D. Mahajan, Stanley A. Schwartz and Madhavan P.N.
Nair
[Abstract] [Full Text] [PDF]
Use of Site-Specifically Tethered Chemical
Nucleases to Study Macromolecular Reactions Srabani Mukherjee
and Rui Sousa
[Abstract] [Full Text] [PDF]
Application of a Colorimetric Assay to
Identify Putative Ribofuranosylaminobenzene 5'-Phosphate Synthase
Genes Expressed with Activity in Escherichia coli Matthew
E. Bechard, Sonya Chhatwal, Rosemarie E. Garcia and Madeline E.
Rasche
[Abstract] [Full Text] [PDF]
Evaluation of intra- and interspecific divergence of satellite
DNA sequences by nucleotide frequency calculation and pairwise
sequence comparison Mikio Kato
[Abstract] [Full Text] [PDF]
A targeted extracellular approach for recording long-term
firing patterns of excitable cells: a practical guide
Craig S. Nunemaker, R. Anthony DeFazio and Suzanne M. Moenter
[Abstract] [Full Text] [PDF]
Optimization of Naked DNA Delivery for Interferon Subtype
Immunotherapy in Cytomegalovirus Infection Emmalene J.
Bartlett, Vanessa S. Cull, Eva N. Mowe, Josephine P. Mansfield
and Cassandra M. James
[Abstract] [Full Text] [PDF]
Progressive Rearrangement of Telomeric Sequences Added
to Both the ITR Ends of the Yeast Linear pGKL Plasmid
Norio Gunge, Hideki Takata, Akira Matsuura and Kohsai Fukuda
[Abstract] [Full Text] [PDF]
A semi-quantitative RT-PCR method to measure the in vivo
effect of dietary conjugated linoleic acid on porcine muscle PPAR
gene expression W.J. Meadus
[Abstract] [Full Text] [PDF]
Expression, Purification and Characterization of Ricin
vectors used for exogenous antigen delivery into the MHC Class
I presentation pathway Daniel C. Smith, Catherine J. Marsden,
J. Michael Lord and Lynne M. Roberts
[Abstract] [Full Text] [PDF]
Experimental Assessment of the Role of Acetaldehyde in
Alcoholic Cardiomyopathy Nicholas S. Aberle II and Jun
Ren
[Abstract] [Full Text] [PDF]
Vol.
4 No. 1
Aequorin-based measurements of intracellular Ca2+-signatures
in plant cells Axel Mithöfer and Christian Mazars
[Abstract] [Full Text] [PDF]
A proteomic approach based on peptide affinity chromatography,
2-dimensional electrophoresis and mass spectrometry to identify
multiprotein complexes interacting with membrane-bound receptors
Carine Bécamel, Nathalie Galéotti, Joël Poncet, Patrick Jouin,
Aline Dumuis, Joël Bockaert and Philippe Marin
[Abstract] [Full Text] [PDF]
A Sensitive Quantification of HHV-6B by Real-time PCR
Bodil Řster and Per Höllsberg
[Abstract] [Full Text] [PDF]
Use of an Immobilized Monoclonal Antibody to Examine Integrin
α5β1 Signaling Independent of Cell Spreading
Wenjie Bao and Staffan Strömblad
[Abstract] [Full Text] [PDF]
PCR-based detection of a rare linear DNA in cell culture
Sergei V. Saveliev
[Abstract] [Full Text] [PDF]
A Method for Analyzing the Ubiquitination and Degradation
of Aurora-A Andrea Klotzbucher, Gaetan Pascreau, Claude
Prigent and Yannick Arlot-Bonnemains
[Abstract] [Full Text] [PDF]
Primary Culture and mRNA Analysis of Human Ovarian Cells
Lesley D. Dunfield, Trevor G. Shepherd and Mark W. Nachtigal
[Abstract] [Full Text] [PDF]
An Efficient Ligation Method in the Making of an in vitro
Virus for in vitro Protein Evolution Ichiro Tabuchi, Sayaka
Soramoto, Miho Suzuki, Koichi Nishigaki, Naoto Nemoto and Yuzuru
Husimi
[Abstract] [Full Text] [PDF]
Methods for Analysis of Matrix Metalloproteinase Regulation
of Neutrophil-Endothelial Cell Adhesion Carlos Fernandez-Patron,
Christine Zouki, Randy M. Whittal, John S.D. Chan, Sandra T. Davidge
and János G. Filep
[Abstract] [Full Text] [PDF]
High-Yield Method for Isolation and Culture of Endothelial
Cells from Rat Coronary Blood Vessels Suitable for Analysis of
Intracellular Calcium and Nitric Oxide Biosynthetic Pathways
Silvia Nistri, Luca Mazzetti, Paola Failli and Daniele Bani
[Abstract] [Full Text] [PDF]
Mouse Aortic Ring Assay: A New Approach of the Molecular
Genetics of Angiogenesis Véronique Masson, Laetitia Devy,
Christine Grignet-Debrus, Sarah Bernt, Khalid Bajou, Silvia Blacher,
Guy Roland, Yawen Chang, Timothy Fong, Peter Carmeliet, Jean-Michel
Foidart and Agnčs Noël
[Abstract] [Full Text] [PDF]
Isolation, growth and identification of colony-forming
cells with erythroid, myeloid, dendritic cell and NK-cell potential
from human fetal liver Marcus O. Muench, David L. Suskind
and Alicia Bárcena
[Abstract] [Full Text] [PDF]
Use of an Anaerobic Chamber Environment for the Assay
of Endogenous Cellular Protein-Tyrosine Phosphatase Activities
Li Zhu and Barry Goldstein
[Abstract] [Full Text] [PDF]
Vol.
3 No. 1
Detection of Progeny Immune Responses after Intravenous
Administration of DNA Vaccine to Pregnant Mice Ke-Qin
Xin, Shin Sasaki, Yoshitsugu Kojima, Nao Jounai, Yasuko Kumamoto,
Kumiko Hashimoto, Kaori Shinoda, Kenji Hamajima, and Kenji Okuda
[Abstract] [Full Text] [PDF]
Investigation of Neuronal Cell Type-Specific Gene Expression
of Ca2+/Calmodulin-dependent Protein Kinase II
Kazuko Mima, Hitomi Donai, and Takashi Yamauchi
[Abstract] [Full Text] [PDF]
Methods for the study of ionic currents and Ca2+-signals
in isolated colonic crypts Gerhard Schultheiss, Sarah
Lan Kocks and Martin Diener
[Abstract] [Full Text] [PDF]
Promoter analysis by saturation mutagenesis Nitin
S. Baliga
[Abstract] [Full Text] [PDF]
A fluorescence microscopy method for quantifying the detection
of prostaglandin endoperoxide H synthase-1 and CD-41 in MEG-01
cells Douglas J. Franks, Cameron Mroske and Odette Laneuville
[Abstract] [Full Text] [PDF]
Measurement of cardiac mechanical function in isolated
ventricular myocytes from rats and mice by computerized video-based
imaging Jun Ren and Loren E. Wold
[Abstract] [Full Text] [PDF]
Acid glycohydrolases in rat spermatocytes, spermatids
and spermatozoa: enzyme activities, biosynthesis and immunolocalization
Aida Abou-Haila and Daulat R.P. Tulsiani
[Abstract] [Full Text] [PDF]
Semiquantitative RT-PCR analysis to assess the expression
levels of multiple transcripts from the same sample Maria
Marone, Simona Mozzetti, Daniela De Ritis, Luca Pierelli, Giovanni
Scambia
[Abstract] [Full Text] [PDF]
Cytochemical techniques and energy-filtering transmission
electron microscopy applied to the study of parasitic protozoa
Marcos A. Vannier-Santos, Ulysses Lins
[Abstract] [Full Text] [PDF]
A sensitive non-radioactive in situ hybridization
method for the detection of chicken IgG g-chain mRNA: a technique
suitable for detecting of variety of mRNAs in tissue sections
Weiming Zheng, Junichi Izaki, Shuichi Furusawa, Yukinori Yoshimura
[Abstract] [Full Text] [PDF]
Vol.
2 No. 1
Post-Electrophoretic Identification of Oxidized Proteins
Craig C. Conrad, John M. Talent, Christina A. Malakowsky,
Robert W. Gracy
[Abstract] [Full Text] [PDF]
Yeast Two-Hybrid: State of the Art Wim Van Criekinge,
Rudi Beyeart
[Abstract] [Full Text] [PDF]
Vol.
1 No. 3
The method of contact angle measurements and estimation
of work of adhesion in bioleaching of metals Aleksandra
Sklodowska, Marek Wozniak, Renata Matlakowska
[Abstract] [Full Text] [PDF]
Role of a Transbilayer pH Gradient in the Membrane Fusion
Activity of the Influenza Virus Hemagglutinin: Use of the R18
Assay to Monitor Membrane Merging
Joăo Ramalho-Santos, Maria C. Pedroso de Lima
[Abstract] [Full Text] [PDF]
Special Feature: Science through the Internet: Researching,
Evaluating and Citing Websites
L.E.J. Lee, E. Misser
[Abstract] [Full Text] [PDF]
Vol.
1 No. 2
Determining Mitomycin C in aqueous solutions and in urine
Dolores Marín, Pedro Perez, Carmen Teijeiro, Emil Palecek
[Abstract] [Full Text] [PDF]
Vol.
1 No. 1
A Method for Assaying Deubiquitinating Enzymes
Jae Il Lee, Seung Kyoon Woo, Keun Il Kim, Kyung Chan Park, Sung
Hee Baek, Yung Joon Yoo, Chin Ha Chung
[Abstract] [Full Text] [PDF]
Coupling Optical and Electrical Measurements in Membranes
Duclohier H., O. Helluin, E. Lea, A.R. Mackie, S. Ladha
[Abstract] [Full Text] [PDF]
Expression and purification of a P-ATPase in E. coli
Geisler, M.
[Abstract] [Full
Text] [PDF]
Liquid-phase synthesis of PEG-conjugated oligonucleotides
Bonora, B.M., S. Zaramella, F.M. Veronese
[Abstract] [Full
Text] [PDF]
Methods for Kluyveromyces lactis b-galactosidase purification
M. Becerra, E. Cerdán, M.I. González Siso
[Abstract] [Full
Text] [PDF]
Microbial DNA extraction from soil for PCR amplification
Yeates,C., M.R.Gillings, A.D.Davison, N.Altavilla, D.A.Veal
[Abstract] [Full
Text] [PDF]
Predicting Intestinal Drug Absorbability and Metabolizability
Mizuma, T., S. Awazu
[Abstract] [Full
Text] [PDF]
Methods for the Detection of D-Amino-Acid Oxidase
Konno, R.
[Abstract] [Full
Text] [PDF]
Measurement of a Bacterial Enzyme Activity in Cell Lysates
Burns, B.P., G. Mendz, S. Hazell
[Abstract] [Full
Text] [PDF]
Yeast Exoglycoproteins as Cryoprotective Agents
Breierova, E.
[Abstract] [Full
Text] [PDF]
Deleterious-Mutation Parameters in Natural Daphnia Populations
Deng, H-W.
[Abstract] [Full
Text] [PDF]
Biological Procedures Online: An Electronic Techniques Journal
Serving Biochemists and Biologists
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