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Author: Gallup, JM
Author: Ackermann, MR
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Methods Article

Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

Jack M. Gallup1* and Mark R. Ackermann1

1 Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA.

* To whom correspondence should be addressed: Jack M. Gallup, Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA. Phone: 1-515-294-5423. Fax: 1-515-294-5844. Email: eag@iastate.edu

Biol. Proced. Online 2006;8:87-152. doi:10.1251/bpo122
Submitted: May 02, 2006; Accepted: July 10, 2006; Published: September 15, 2006.

Indexing terms: Reverse Transcription; DNA, Complementary.


Figure 16 Enlarged

Fig. 16:

Portion of FF2-6-001 qPCR set-up tool MasterEntrySheet.xls file (Sheet 1) which allows the user to define Test Plate parameters (and correct master mix usage), Sample Plate parameters (and correct master mix usage), and NRC Plate parameters (and correct master mix usage).

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