Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

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Author: Gallup, JM
Author: Ackermann, MR
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Methods Article

Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

Jack M. Gallup¹^* and Mark R. Ackermann¹

¹ Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA.

* To whom correspondence should be addressed: Jack M. Gallup, Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA. Phone: 1-515-294-5423. Fax: 1-515-294-5844. Email: eag@iastate.edu

Biol. Proced. Online 2006;8:87-152. doi:10.1251/bpo122
Submitted: May 02, 2006; Accepted: July 10, 2006; Published: September 15, 2006.

Indexing terms: Reverse Transcription; DNA, Complementary.

Figure 33 Enlarged

Fig. 33:

Portion of FF2-6-001 qPCR set-up tool MasterEntrySheet.xls file (Sheet 2) which shows the automatically-calculated master mix set-ups for all final target Sample Plates. ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit and the ABI GeneAmp® 5700 Sequence Detection System were used here. All master mix calculation files automatically figure in safe extra preparative amounts so the user will not run short on final volumes or run into bubbles at the bottom of sample tubes.

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