Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

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Author: Gallup, JM
Author: Ackermann, MR
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Methods Article

Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions

Jack M. Gallup¹^* and Mark R. Ackermann¹

¹ Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA.

* To whom correspondence should be addressed: Jack M. Gallup, Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University. Ames, Iowa 50011-1250. USA. Phone: 1-515-294-5423. Fax: 1-515-294-5844. Email: eag@iastate.edu

Biol. Proced. Online 2006;8:87-152. doi:10.1251/bpo122
Submitted: May 02, 2006; Accepted: July 10, 2006; Published: September 15, 2006.

Indexing terms: Reverse Transcription; DNA, Complementary.

Figure 13 Enlarged

Fig. 13:

Key portion of FF2-6-001 qPCR set-up tool MasterEntrySheet.xls file (Sheet 1) wherein the user enters numerous other numerically descriptive parameters which define the particular qPCR set-up and/or approach being pursued.

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