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Author: Uribe, S
Author: Sampedro, JG
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Methods Article

Measuring Solution Viscosity and its Effect on Enzyme Activity

Salvador Uribe2 and José G. Sampedro1*

1 Área Académica de Nutrición, Instituto de Ciencias de la Salud ICSA, Universidad Autónoma del Estado de Hidalgo (UAEH). Abasolo 600, C.P 42000. Pachuca, Hidalgo. Mexico.
2 Departamento de Bioquímica, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM). A.P. 70-242, 04510 Mexico City. Mexico.

* To whom correspondence should be addressed: José G. Sampedro, Área Académica de Nutrición, Instituto de Ciencias de la Salud ICSA, Universidad Autónoma del Estado de Hidalgo (UAEH). Abasolo 600, C.P 42000. Pachuca, Hidalgo. Mexico. Phone: (5277) 1717 2000 ext. 5102. Fax: (5277) 1717 2000 ext. 5111. Email: jsamped@ifisiol.unam.mx

Biol. Proced. Online 2003;5:108-115. doi:10.1251/bpo52
Submitted: January 17, 2003; Accepted: March 29, 2003; Published: May 01, 2003.

Indexing terms: Viscosity; Trehalose.

Abbreviations: EGTA, Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N’,N’,-tetraacetic acid; PIPES, Piperazine-N,N’-bis(2-ethanesulfonic acid); Tris, Tris(hydroxymethyl)aminomethane; SDS-PAGE, Sodium-dodecyl-sulphate-Polyacrylamide Gel Electrophoresis; YPD, Yeast-Peptone-Dextrose.

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Fig. 1 Enlarged

Fig. 1:

A: Effect of solute concentration on medium viscosity. The viscosity (η) at a given concentration of carbohydrate (expressed as mass fraction, c) was measured in a falling ball viscometer at 20°C as described in methods. B: Solution viscosity (η) inhibits the plasma membrane H+-ATPase. The Vmax at a given concentration of solute was calculated by measuring the rate of ATP hydrolysis versus ATP concentration and fitting to the Hill equation [eq 2]. The relative Vmax (Vmax0/Vmax) was plotted against the relative viscosity (η/η0). Both, η0 and Vmax0 are the solution viscosity and the maximum velocity respectively in the absence of the viscosogenic agent: Glycerol ○; Trehalose ●; Sucrose ‌.

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ISSN: 1480-9222
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