The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

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Author: Harrison, JJ
Author: Turner, RJ
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Methods Article

The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

Joe J. Harrison², Howard Ceri², Jerome Yerly³, Carol A. Stremick², Yaoping Hu³, Robert Martinuzzi⁴ and Raymond J. Turner¹^*

¹ Department of Biological Sciences, University of Calgary. T2N 1N4. Canada.
² Department of Biological Sciences and Biofilm Research Group, University of Calgary. T2N 1N4. Canada.
³ Electrical and Computer Engineering, Schulich School of Engineering, University of Calgary. T2N 1N4. Canada.
⁴ Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary. 2500 University Drive N.W., Calgary, AB T2N 1N4. Canada.

* To whom correspondence should be addressed: Raymond J. Turner, Department of Biological Sciences, University of Calgary. T2N 1N4. Canada. Phone: 403-220-4308. Email: turnerr@ucalgary.ca

Biol. Proced. Online 2006;8:194-215. doi:10.1251/bpo127
Submitted: September 06, 2006; Accepted: October 28, 2006; Published: December 19, 2006.

Indexing terms: Biofilms; Imaging, Three-Dimensional.

Figure 2 Enlarged

Fig. 2:

SEM of bacterial biofilms grown in the CBD. For the sake of comparison, the biofilms were grown in either rich or minimal medium (as summarized in Table 1) and then fixed using 1 of 2 different protocols. These micrographs were chosen to illustrate that medium composition has an impact on the capacity of bacteria to form biofilms, which further varies between genus, species and strains. Moreover, the choice of fixing protocols influences how well microstructures may be preserved, which may impact on the interpretation of SEM data.

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