The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

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Author: Harrison, JJ
Author: Turner, RJ
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Methods Article

The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

Joe J. Harrison², Howard Ceri², Jerome Yerly³, Carol A. Stremick², Yaoping Hu³, Robert Martinuzzi⁴ and Raymond J. Turner¹^*

¹ Department of Biological Sciences, University of Calgary. T2N 1N4. Canada.
² Department of Biological Sciences and Biofilm Research Group, University of Calgary. T2N 1N4. Canada.
³ Electrical and Computer Engineering, Schulich School of Engineering, University of Calgary. T2N 1N4. Canada.
⁴ Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary. 2500 University Drive N.W., Calgary, AB T2N 1N4. Canada.

* To whom correspondence should be addressed: Raymond J. Turner, Department of Biological Sciences, University of Calgary. T2N 1N4. Canada. Phone: 403-220-4308. Email: turnerr@ucalgary.ca

Biol. Proced. Online 2006;8:194-215. doi:10.1251/bpo127
Submitted: September 06, 2006; Accepted: October 28, 2006; Published: December 19, 2006.

Indexing terms: Biofilms; Imaging, Three-Dimensional.

Figure 3 Enlarged

Fig. 3:

CLSM of AO stained bacterial biofilms grown in the CBD. The images on the left are 2D averages of image z-stacks, whereas the images on the right are isosurface rendered 3D visualizations of the same data set (prepared using Leica® Confocal Software). These data sets illustrate that mature biofilms may adopt a number of structures that are distinct from the archetypal “stalk and mushroom” microcolony structures that are well characterized for P. aeruginosa. Each panel represents a square surface area of approximately 238 × 238 μm.

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