The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

Display

Search in Pubmed

Author: Harrison, JJ
Author: Turner, RJ
Related works

Methods Article

The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device

Joe J. Harrison², Howard Ceri², Jerome Yerly³, Carol A. Stremick², Yaoping Hu³, Robert Martinuzzi⁴ and Raymond J. Turner¹^*

¹ Department of Biological Sciences, University of Calgary. T2N 1N4. Canada.
² Department of Biological Sciences and Biofilm Research Group, University of Calgary. T2N 1N4. Canada.
³ Electrical and Computer Engineering, Schulich School of Engineering, University of Calgary. T2N 1N4. Canada.
⁴ Mechanical and Manufacturing Engineering, Schulich School of Engineering, University of Calgary. 2500 University Drive N.W., Calgary, AB T2N 1N4. Canada.

* To whom correspondence should be addressed: Raymond J. Turner, Department of Biological Sciences, University of Calgary. T2N 1N4. Canada. Phone: 403-220-4308. Email: turnerr@ucalgary.ca

Biol. Proced. Online 2006;8:194-215. doi:10.1251/bpo127
Submitted: September 06, 2006; Accepted: October 28, 2006; Published: December 19, 2006.

Indexing terms: Biofilms; Imaging, Three-Dimensional.

Figure 5 Enlarged

Fig. 5:

TRITC-conjugated lectin staining of C. tropicalis and B. cenocepacia biofilms. A. An overlay image of a C. tropicalis biofilm that was stained with AO (green emission) and TRITC-ConA (red emission). B. AO staining of the section illustrated in panel A. C. TRITC-ConA staining of the section illustrated in panel A. D. An overlay image of a B. cenocepacia biofilm that was stained with Syto-9 and TRITC-PNA. E. Syto-9 staining of the section illustrated in panel D. F. TRITC-PNA staining of the section illustrated in panel D. Each panel represents a square surface area of approximately 238 × 238 μm.

Disclaimer/Legal Copyright Privacy Policy

ISSN: 1480-9222
Article © by the author(s). This paper is Open Access and is published in Biological Procedures Online under license from the author(s). Copying, printing, redistribution and storage permitted. Journal © 1997-2006 Biological Procedures Online.