Vol. 8 No. 1

The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device Joe J. Harrison, Howard Ceri, Jerome Yerly, Carol A. Stremick, Yaoping Hu, Robert Martinuzzi and Raymond J. Turner
[Abstract]     [Full Text]     [PDF]

Microarray validation: factors influencing correlation between oligonucleotide microarrays and real-time PCR Jeanine S. Morey, James C. Ryan and Frances M. Van Dolah
[Abstract]     [Full Text]     [PDF]

A statistical framework for genetic association studies of power curves in bird flight Min Lin, Wei Zhao and Rongling Wu
[Abstract]     [Full Text]     [PDF]

Techniques of EMG signal analysis: detection, processing, classification and applications (Correction) M.B.I. Raez, M.S. Hussain and F. Mohd-Yasin
[Abstract]     [Full Text]     [PDF]

Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system James R. Emmerson, David L. Gally and Andrew J. Roe
[Abstract]     [Full Text]     [PDF]

Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions Jack M. Gallup and Mark R. Ackermann
[Abstract]     [Full Text]     [PDF]

Combining genetic and biochemical approaches to identify functional molecular contact points Matthew P. Badtke, Feng Cao and John E. Tavis
[Abstract]     [Full Text]     [PDF]

Chromatin immunoprecipitation assay detects ERα recruitment to gene specific promoters in uterus Sanhita Ray and Sanjoy K. Das
[Abstract]     [Full Text]     [PDF]

Practical three color live cell imaging by widefield microscopy Jianrun Xia, Song Hon H. Kim, Susan Macmillan and Ray Truant
[Abstract]     [Full Text]     [PDF]

Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits HSV-2 mediated mortality Bobbie Ann Austin, Cassandra M. James, Peter Härle and Daniel J.J. Carr
[Abstract]     [Full Text]     [PDF]

Use of chromatin immunoprecipitation (ChIP) to detect transcription factor binding to highly homologous promoters in chromatin isolated from unstimulated and activated primary human B cells Rebecca L. Dryer and Lori R. Covey
[Abstract]     [Full Text]     [PDF]

Cell-based expression cloning for identification of polypeptides that hypersensitize mammalian cells to mitotic arrest Hiroshi Y. Yamada and Gary J. Gorbsky
[Abstract]     [Full Text]     [PDF]

Techniques of EMG signal analysis: detection, processing, classification and applications M.B.I. Raez, M.S. Hussain and F. Mohd-Yasin
[Abstract]     [Full Text]     [PDF]

Rapid and simple comparison of messenger RNA levels using real-time PCR Andrée-Anne Dussault and Marc Pouliot
[Abstract]     [Full Text]     [PDF]

Vol. 7 No. 1

Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines Jose M. Mellado-Gil and Manuel Aguilar-Diosdado
[Abstract]     [Full Text]     [PDF]

Use of three-dimensional collagen gels to study mechanotransduction in T47D breast epithelial cells Michele A. Wozniak and Patricia J. Keely
[Abstract]     [Full Text]     [PDF]

Analyzing folding and degradation of metabolically labelled polypeptides by conventional and diagonal sodium dodecyl sulfate-polyacrylamide gel electrophoresis Tatiana Soldà, Silvia Olivari and Maurizio Molinari
[Abstract]     [Full Text]     [PDF]

Comparative proteomics using 2-D gel electrophoresis and mass spectrometry as tools to dissect stimulons and regulons in bacteria with sequenced or partially sequenced genomes Sergio Encarnación, Magdalena Hernández, Gabriel Martínez-Batallar, Sandra Contreras, María del Carmen Vargas and Jaime Mora
[Abstract]     [Full Text]     [PDF]

A guideline for analyzing circadian wheel-running behavior in rodents under different lighting conditions Corinne Jud, Isabelle Schmutz, Gabriele Hampp, Henrik Oster and Urs Albrecht
[Abstract]     [Full Text]     [PDF]

Analyzing ligation mixtures using a PCR based method Prafulla K. Chandra and Stephen K. Wilek
[Abstract]     [Full Text]     [PDF]

New quick method for isolating RNA from laser captured cells stained by immunofluorescent immunohistochemistry; RNA suitable for direct use in fluorogenic TaqMan one-step real-time RT-PCR Jack M. Gallup, Kenji Kawashima, Ginger Lucero and Mark R. Ackermann
[Abstract]     [Full Text]     [PDF]

Easy detection of chromatin binding proteins by the histone association assay Robin Ricke and Anja-Katrin Bielinsky
[Abstract]     [Full Text]     [PDF]

In vitro and in vivo assays for osteoclast apoptosis Toru Akiyama, Tsuyoshi Miyazaki, Phillippe Bouillet, Kozo Nakamura, Andreas Strasser and Sakae Tanaka
[Abstract]     [Full Text]     [PDF]

Involvement of integrin-linked kinase in capillary/tube-like network formation of human vascular endothelial cells Motomu Watanabe, Yayoi Fujioka-Kaneko, Hisayuki Kobayashi, Mamoru Kiniwa, Michihiko Kuwano and Yuji Basaki
[Abstract]     [Full Text]     [PDF]

An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carrier, ion-selective microelectrodes Jason S.T. Deveau, Michael I. Lindinger and Bernard Grodzinski
[Abstract]     [Full Text]     [PDF]

Rapid cryopreservation of five mammalian and one mosquito cell line at -80°C while attached to flasks in a serum free cryopreservative Joe Corsini and Ethan Mann
[Abstract]     [Full Text]     [PDF]

  Inactivation of sortilin (a novel lysosomal sorting receptor) by dominant negative competition and RNA interference Stephane Lefrancois, Maryssa Canuel, Jibin Zeng and Carlos R. Morales
[Abstract]     [Full Text]     [PDF]

  An in vitro method to select malignant cells from surgical biopsies of breast cancer patients Kelly E. Corcoran, Prem S. Patel and Pranela Rameshwar
[Abstract]     [Full Text]     [PDF]

  A rapid and sensitive assay for quantification of siRNA efficiency and specificity Nicola Smart, Peter James Scambler and Paul Richard Riley
[Abstract]     [Full Text]     [PDF]

  Construction of a bacterial autoinducer detection system in mammalian cells Erin K. Shiner, Sheila Reddy, Cody Timmons, Guigen Li, Simon C. Williams and Kendra P. Rumbaugh
[Abstract]     [Full Text]     [PDF]

  A simple and non-radioactive technique to study the effect of monophosphoesters on matrix vesicle-mediated calcification Rama Garimella, Joseph B. Sipe and H. Clarke Anderson
[Abstract]     [Full Text]     [PDF]

  Synthesis, purification and crystallization of guanine-rich RNA oligonucleotides Baocheng Pan, Ke Shi and Muttaiya Sundaralingam
[Abstract]     [Full Text]     [PDF]

  The use of antisense oligonucleotides in evaluating survivin as a therapeutic target for radiation sensitization in lung cancer Eric T. Shinohara, Dennis E. Hallahan and Bo Lu
[Abstract]     [Full Text]     [PDF]

  A method for rapidly screening functionality of actin mutants and tagged actins Heidi Rommelaere, Davy Waterschoot, Katrien Neirynck, Joël Vandekerckhove and Christophe Ampe
[Abstract]     [Full Text]     [PDF]

  Direct determination of unbound lipophilic ligands in aqueous solutions Inge N. Bojesen
[Abstract]     [Full Text]     [PDF]

  Screening of compounds toxicity against human monocytic cell line-THP-1 by flow cytometry Neora Pick, Scott Cameron, Dorit Arad and Yossef Av-Gay
[Abstract]     [Full Text]     [PDF]

  Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins Renee L. Shirley, M. Rachel Richards and Michael R. Culbertson
[Abstract]     [Full Text]     [PDF]

  Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library Jingzhi Li and Bingdong Sha
[Abstract]     [Full Text]     [PDF]

  Methods for analyzing the role of DNA methylation and chromatin structure in regulating T lymphocyte gene expression Qianjin Lu and Bruce Richardson
[Abstract]     [Full Text]     [PDF]

  Analysis of among-site variation in substitution patterns Neeraja M. Krishnan, Sameer Z. Raina and David D. Pollock
[Abstract]     [Full Text]     [PDF]

  A novel method to analyze leukocyte rolling behavior in vivo Jessica L. Dunne, Adam P. Goobic, Scott T. Acton and Klaus Ley
[Abstract]     [Full Text]     [PDF]

  A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1 Reena Pinhero, Peter Liaw and Krassimir Yankulov
[Abstract]     [Full Text]     [PDF]

  An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes Michiel Vermeulen and Hendrik G. Stunnenberg
[Abstract]     [Full Text]     [PDF]

  Visualizing the needle in the haystack: In situ hybridization with fluorescent dendrimers Jacquelyn Gerhart, Michael Baytion, Jordanna Perlman, Christine Neely, Bridget Hearon, Thor Nilsen, Robert Getts, James Kadushin and Mindy George-Weinstein
[Abstract]     [Full Text]     [PDF]

  Two different point mutations in ABL gene ATP-binding domain conferring primary imatinib resistance in a Chronic Myeloid Leukemia (CML) patient: A case report Zafar Iqbal, Rubina T. Siddiqui and Javed A. Qureshi
[Abstract]     [Full Text]     [PDF]

  Use of ion-channel modulating agents to study cyanobacterial Na⁺ - K⁺ fluxes Francesco Pomati, Brendan P. Burns and Brett A. Neilan
[Abstract]     [Full Text]     [PDF]

  Flow cytometry based techniques to study testicular acidophilic granulocytes from the protandrous fish gilthead seabream (Sparus aurata L.) Elena Chaves-Pozo, Victoriano Mulero, José Meseguer and Alfonsa García Ayala
[Abstract]     [Full Text]     [PDF]

  Dynamic force microscopy for imaging of viruses under physiological conditions Ferry Kienberger, Rong Zhu, Rosita Moser, Christian Rankl, Dieter Blaas and Peter Hinterdorfer
[Abstract]     [Full Text]     [PDF]

  Using C. elegans to screen for targets of ethanol and behavior-altering drugs Andrew G. Davies and Steven L. McIntire
[Abstract]     [Full Text]     [PDF]

  Expression and purification of recombinant vesicular glutamate transporter VGLUT1 using PC12 cells and high five insect cells Søren S.L. Andersen
[Abstract]     [Full Text]     [PDF]

  Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells Shirin Munir, Sushmita Singh, Kuljeet Kaur and Vivek Kapur
[Abstract]     [Full Text]     [PDF]

  Detection of hCG responsive expression of the steroidogenic acute regulatory protein in mouse leydig cells Pulak R. Manna, Ilpo T. Huhtaniemi and Douglas M. Stocco
[Abstract]     [Full Text]     [PDF]

  Fluorescence Resonance Energy Transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins Victoria E. Centonze, Beth A. Firulli and Anthony B. Firulli
[Abstract]     [Full Text]     [PDF]

  Recombinant antigens for immunodiagnosis of cystic echinococcosis Jun Li, Wen-Bao Zhang and Donald P. McManus
[Abstract]     [Full Text]     [PDF]

  Serum-free cryopreservation of five mammalian cell lines in either a pelleted or suspended state Joe Corsini, Christy Hacker and Charles Bare
[Abstract]     [Full Text]     [PDF]

  Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system Hans-Jürgen Stark, Axel Szabowski, Norbert E. Fusenig and Nicole Maas-Szabowski
[Abstract]     [Full Text]     [PDF]

  Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering Ayman Antoun, Michael Y. Pavlov, Tanel Tenson and Måns Ehrenberg
[Abstract]     [Full Text]     [PDF]

  Negative staining and image classification – powerful tools in modern electron microscopy Melanie Ohi, Ying Li, Yifan Cheng and Thomas Walz
[Abstract]     [Full Text]     [PDF]

  Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines Beth A. Firulli, David M. Virshup and Anthony B. Firulli
[Abstract]     [Full Text]     [PDF]

  Experimental modulation of capsule size in Cryptococcus neoformans Oscar Zaragoza and Arturo Casadevall
[Abstract]     [Full Text]     [PDF]

  Force and compliance measurements on living cells using Atomic Force Microscopy (AFM) Ewa P. Wojcikiewicz, Xiaohui Zhang and Vincent T. Moy
[Abstract]     [Full Text]     [PDF]

  A method to identify p62’s UBA domain interacting proteins Julia W. Pridgeon, Thangiah Geetha and Marie W. Wooten
[Abstract]     [Full Text]     [PDF]

  Tumor cell-collagen interactions: identification and semi-quantitative evaluation of selectively-expressed genes by combination of differential display- and multiplex-PCR Rosalia Sirchia, Valentina Ciacciofera and Claudio Luparello
[Abstract]     [Full Text]     [PDF]

  T cell integrin overexpression as a model of murine autoimmunity Raymond L. Yung, Donna Ray, Ru Ran Mo and Jun Chen
[Abstract]     [Full Text]     [PDF]

  A screen to identify drug resistant variants to target-directed anti-cancer agents Mohammad Azam, Tal Raz, Valentina Nardi, Sarah L. Opitz and George Q. Daley
[Abstract]     [Full Text]     [PDF]

  Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes Bianca Obermaier, Marc Dauer, Jan Herten, Katharina Schad, Stefan Endres and Andreas Eigler
[Abstract]     [Full Text]     [PDF]

  Swarming populations of Salmonella represent a unique physiological state coupled to multiple mechanisms of antibiotic resistance Wook Kim and Michael G. Surette
[Abstract]     [Full Text]     [PDF]

  Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects Supriya D. Mahajan, Ravikumar Aalinkeel, Stanley A. Schwartz, Ram P. Chawda and Madhavan P.N. Nair
[Abstract]     [Full Text]     [PDF]

  Use of surface plasmon resonance for the measurement of low affinity binding interactions between HSP72 and measles virus nucleocapsid protein Xinsheng Zhang and Michael Oglesbee
[Abstract]     [Full Text]     [PDF]

  Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae Troy A. A. Harkness, Terra G. Arnason, Charmaine Legrand and Ashley Lone
[Abstract]     [Full Text]     [PDF]

  Dissecting mitosis by RNAi in Drosophila tissue culture cells Helder Maiato, Claudio E. Sunkel and William C. Earnshaw
[Abstract]     [Full Text]     [PDF]

  Two-dimensional DNA displays for comparisons of bacterial genomes Chad Malloff, Edie Dullaghan, Alice Li, Richard Stokes, Rachel Fernandez and Wan Lam
[Abstract]     [Full Text]     [PDF]

  Application of 4,5-diaminofluorescein to reliably measure nitric oxide released from endothelial cells in vitro Thomas R. Räthel, Jürgen F. Leikert, Angelika M. Vollmar and Verena M. Dirsch
[Abstract]     [Full Text]     [PDF]