Direct determination of unbound lipophilic ligands in aqueous solutions

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Author: Bojesen, IN
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Direct determination of unbound lipophilic ligands in aqueous solutions

Inge N. Bojesen¹^*

¹ Department of Medical Biochemistry and Genetics, Lab. B., University of Copenhagen, The Panum Institute. Blegdamsvej 3, DK-2200 Copenhagen N. Denmark.

* To whom correspondence should be addressed: Inge N. Bojesen, Department of Medical Biochemistry and Genetics, Lab. B., University of Copenhagen, The Panum Institute. Blegdamsvej 3, DK-2200 Copenhagen N. Denmark. Email: norby@imbg.ku.dk

Biol. Proced. Online 2004;6:226-234. doi:10.1251/bpo93
Submitted: March 02, 2004; Accepted: September 26, 2004; Published: October 20, 2004.

Indexing terms: Erythrocyte membrane; Ligands; Binding sites.

Figure 1 Enlarged

Fig. 1:

The relation between supernatant 3H-ligand concentrations including ligand bound to dissolved binding capacity and the reciprocal value of the four dilution factors. A: Experimental data of anandamide at 37°C and ν = 0.148, with 0.1% BSA-filled ghosts. Regression line is y = 25.07 ± 0.54 x + 9.67 ± 0.14, r = 0.999, P = 0.00046. B: Experimental data of arachidonate at 38°C and ν = 0.019, with 0.2% BSA-filled ghosts. Regression line is y = 0.442 ± 0.031 x + 0.203 ± 0.008, r = 0.995, P = 0.00489.

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