Direct determination of unbound lipophilic ligands in aqueous solutions

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Author: Bojesen, IN
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Direct determination of unbound lipophilic ligands in aqueous solutions

Inge N. Bojesen¹^*

¹ Department of Medical Biochemistry and Genetics, Lab. B., University of Copenhagen, The Panum Institute. Blegdamsvej 3, DK-2200 Copenhagen N. Denmark.

* To whom correspondence should be addressed: Inge N. Bojesen, Department of Medical Biochemistry and Genetics, Lab. B., University of Copenhagen, The Panum Institute. Blegdamsvej 3, DK-2200 Copenhagen N. Denmark. Email: norby@imbg.ku.dk

Biol. Proced. Online 2004;6:226-234. doi:10.1251/bpo93
Submitted: March 02, 2004; Accepted: September 26, 2004; Published: October 20, 2004.

Indexing terms: Erythrocyte membrane; Ligands; Binding sites.

Figure 2 Enlarged

Fig. 2:

The relationship between ligand water-phase concentration [L] and molar ratio of ligand to BSA (ν) presented according to (Eq.3). A: Anandamide data at 23°C, pH 7.3. The regression line is y = 0.945 ± 0.119 x + 28.72 ± 1.689, r = 0.95, P = 0.00012. B: Arachidonate data at 10°C, pH 7.3. The regression line is y = 0.308 ± 0.082 x + 2.586 ± 0.231, r = 0.71, P = 0.0021.

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