Laboratory Biosafety Guidelines
2nd Edition 1996

Table Of Contents

1. INTRODUCTION:
1.1 Preface
1.2 Scope of the document

2. CONTAINMENT OF BIOHAZARDS:
2.1 Introduction
2.2 Safety Practices
2.3 Use of Laboratory Animals

3. REGULATIONS
3.1 Importation
3.2 Exportation
3.3 Transportation
3.4 The Health of Animals Act 1990
3.4.1 Non-Indigenous viruses (Table 1)

4. CLASSIFICATION OF BIOLOGICAL AGENTS ACCORDING TO RISK:
4.1 General
4.2 Recombinant DNA and Genetic Manipulation
4.3 Use of Mammalian Cells in Culture
4.4 Risk Levels Associated with the Use of Laboratory Animals
4.5 Criteria for Classification of Biological Agents by Risk Group
4.6 Categories of Pathogens:
4.6.1 Risk Group 1 Agents
4.6.2 Risk Group 2 Agents
4.6.3 Risk Group 3 Agents
4.6.4 Risk Group 4 Agents

5. PHYSICAL CONTAINMENT LEVELS:
5.1 Containment Level 1
5.1.1 Physical Requirements
5.1.2 Operational Requirements
5.2 Containment Level 2
5.2.1 Physical Requirements
5.2.2 Operational Requirements
5.3 Containment Level 3
5.3.1 Physical Requirements
5.3.2 Operational Requirements
5.4 Containment Level 4
5.4.1 Physical Requirements
5.4.2 Operational Requirements
5.5 Animal Biohazard Containment Facilities

6. LARGE SCALE PRODUCTION OF MICROORGANISMS:
6.1 Introduction
6.2 Scope
6.3 Special Considerations
6.3.1 Biological Safety Officer
6.3.2 Transgenic Plants and Animals
6.3.3 Cell Lines
6.4 Containment Levels and Practices
6.4.1 Containment Level Large Scale 1 (LS1)
6.4.2 Containment Level Large Scale 2 (LS2)
6.4.3 Containment Level Large Scale 3 (LS3)
6.4.4 Containment Level Large Scale 4 (LS4)
6.5 Transportation of Large Scale Products
6.5.1 Transportation
6.5.2 Transportation On-site Between Containment Areas

7. LABORATORY DESIGN:
7.1 Laboratory Location
7.2 Physical Construction of Containment Barriers
7.3 Air-Handling Systems
7.3.1 Requirements for Biological Safety Cabinets in all Levels of Laboratories
7.3.2 Requirements for HEPA Filters Levels 2-4 Containment Laboratories
7.4 Decontamination, Sterilization and Waste-Disposal Systems
7.5. Personal Hygiene and Safety Facilities
7.6 Building Services
7.7 Emergency and Monitoring Provisions
7.8 Performance, Verification and Testing

APPENDIX A:
Safety Equipment

APPENDIX B:
Biological Safety Cabinets, Fume Hoods and Filtration Systems

APPENDIX C:
Bibliography

APPENDIX D:
Glossary

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